Generator

Part:BBa_K145113:Design

Designed by: Jonas Demeulemeester   Group: iGEM08_KULeuven   (2008-08-08)


Constitutive PoPS -> TetR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Medium-weak promoter chosen combined with a medium RBS in order to have strong repression yet a good induction by aTc.


Source

other parts

References